“In Vitro Neurons Learn and Exhibit Sentience When Embodied in a Simulated Game-World”, Brett J. Kagan, Andy C. Kitchen, Nhi T. Tran, Bradyn J. Parker, Anjali Bhat, Ben Rollo, Adeel Razi, Karl J. Friston2021-12-03 (, ; similar)⁠:

Integrating neurons into digital systems to leverage their innate intelligence may enable performance infeasible with silicon alone, along with providing insight into the cellular origin of intelligence.

We developed DishBrain, a system which exhibits natural intelligence by harnessing the inherent adaptive computation of neurons in a structured environment. In vitro neural networks from human or rodent origins, are integrated with in silico computing via high-density multielectrode array. Through electrophysiological stimulation and recording, cultures were embedded in a simulated game-world, mimicking the arcade game Pong.

Applying a previously untestable theory of active inference via the Free Energy Principle, we found that learning was apparent within 5 minutes of real-time gameplay, not observed in control conditions. Further experiments demonstrate the importance of closed-loop structured feedback in eliciting learning over time.

Cultures display the ability to self-organize in a goal-directed manner in response to sparse sensory information about the consequences of their actions.