“Generation of Genome-Edited Dogs by Somatic Cell Nuclear Transfer”, Dong-Ern Kim, Ji-Hye Lee, Kuk-Bin Ji, Kang-Sun Park, Tae-Young Kil, Okjae Koo, Min-Kyu Kim2022-07-13 (dog cloning, gene editing)⁠:

Background: Canine cloning technology based on somatic cell nuclear transfer (SCNT) combined with genome-editing tools such as CRISPR-Cas9 can be used to correct pathogenic mutations in purebred dogs or to generate animal models of disease.

Results: We constructed a CRISPR-Cas9 vector targeting canine DJ-1. Genome-edited canine fibroblasts were established using vector transfection and antibiotic selection. We performed canine SCNT using genome-edited fibroblasts and successfully generated two genome-edited dogs. Both genome-edited dogs had insertion-deletion mutations at the target locus, and DJ-1 expression was either downregulated or completely repressed.

Conclusion: SCNT successfully produced genome-edited dogs by using the CRISPR-Cas9 system for the first time.

See Also:

• Gene editing restores dystrophin expression in a canine model of Duchenne muscular dystrophy

• CRISPR-Cas9 effectors facilitate generation of single-sex litters and sex-specific phenotypes

• Extensive Mammalian Germline Genome Engineering