“C₆₀ in Olive Oil Causes Light-Dependent Toxicity and Does Not Extend Lifespan in Mice”, Kristopher J. Grohn, Brandon S. Moyer, Danique C. Wortel, Cheyanne M. Fisher, Ellie Lumen, Anthony H. Bianchi, Kathleen Kelly, Paul S. Campbell, Douglas E. Hagrman, Roger G. Bagg, James Clement, Aaron J. Wolfe, Andrea Basso, Cristina Nicoletti, Giovanni Lai, Mauro Provinciali, Marco Malavolta, Kelsey J. Moody2020-10-29 (longevity, animal study methodology; similar)⁠:

C₆₀, a potent antioxidant, has been reported to substantially extend the lifespan of rodents when formulated in olive oil (C₆₀-OO) or extra virgin olive oil (C₆₀-EVOO). Despite there being no regulated form of C₆₀-OO, people have begun obtaining it from online sources and dosing it to themselves or their pets, presumably with the assumption of safety and efficacy.

In this study, we obtain C₆₀-OO from a sample of online vendors, and find marked discrepancies in appearance, impurity profile, concentration, and activity relative to pristine C₆₀-OO formulated in-house. We additionally find that pristine C₆₀-OO causes no acute toxicity in a rodent model but does form toxic species that can cause statistically-significant morbidity and mortality in mice in under 2 weeks when exposed to light levels consistent with ambient light. Intraperitoneal injections of C₆₀-OO did not affect the lifespan of CB6F1 female mice.

Finally, we conduct a lifespan and health span study in males and females C57BL/6 J mice comparing oral treatment with pristine C₆₀-EVOO and EVOO alone versus untreated controls. We failed to observe statistically-significant lifespan and health span benefits of C₆₀-EVOO or EVOO supplementation compared to untreated controls, both starting the treatment in adult or old age.

Our results call into question the biological benefit of C₆₀-OO in aging.

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