“Programmed Chromosome Fission and Fusion Enable Precise Large-Scale Genome Rearrangement and Assembly”, Kaihang Wang, Daniel de la Torre, Wesley E. Robertson, Jason W. Chin2019-08-30 (genome synthesis)⁠:

Programmable genome engineering: The model bacterium Escherichia coli has a single circular chromosome.

Wang et al. created a method to fragment the E. coli genome into independent chromosomes that can be modified, rearranged, and recombined.

The efficient fission of the unmodified E. coli genome into two defined, stable pairs of synthetic chromosomes provides common intermediates for large-scale genome manipulations such as inversion and translocation. Fusion of synthetic chromosomes from distinct cells generated a single genome in a target cell.

Precise, rapid, large-scale genome engineering operations are useful tools for creating diverse synthetic genomes.